Abstract:
In vitro production of bovine embryos comprises a lot of factors that can influence the successful of this technique, oxidative stress being one of them. These factors can influence the evolution of important development processes such as the maternal to zygotic transition and the embryonic genome activation. Adding antioxidants to in vitro culture media exerts the key role to reduce the effects of reactive oxidative species produced during assisted reproduction technique, influencing in a positive way also the early embryonic developement. The objective of this study was to determine the
effect of antioxidant rosmarinic acid (105 μM), added to in vitro bovine oocytes maturation media, on the quality of embryo produced based on gene expression level of OCT4, SOX2, IGF1R and IGF2R. For this purpose, we used 35 bovine ovaries taken from slougtherhouse from which we obtain 202 cumulus-oocyte-complexes and 127 of them were maturated in vitro based on morphological aspects. The cumulus-oocyte-complexes were divided in two groups: control (M1, M2, M3) and with acid rozmarinic (AR1, AR2 and AR3). The levels of OCT4, SOX2, IGF1R and IGF2R were the highest in group AR1, embryos obtain from oocytes class I supplemented with rozmarinic acid, where OCT4 expression was 4.08, SOX2 was 27.66, IGF1R and IGF2R were 53.44 and 25.10.
