Abstract:
A variant of a turbidimetric method of raw fat
determination from seed, which proved to be simple, fast, and accurate, was
described in this paper. The method was based on the extraction of lipids
from 20 mg samples into acetone followed by their treatment with 1.5%
solution of sulfosalicylic acid and spectral measurement at 440 nm against
a blank of the reagents. Suitable volumes of acetone extracts should be
taken for oily seed samples such as flax, sunflower, or soybean. A standard
curve was made with raw fat extracted from the species being analysed. For
analysing a large number of samples, the standardization of the
turbidimetric method with a few representative Soxhlet values was
recommended. Possible interferences, as well as the real results obtained
within large scale analyses ,are also shown.
